"To investigate the effect of Chinese herbs for nourishing yin and removing fire (NYRF) on gene expressions of estrogen receptor alpha (ER alpha), insulin-like growth factor-1 receptor (IGF-1R) and epithelial growth factor receptor (EGFR) in the epiphyseal growth plate of the female pubertal rats.
The rats were randomly divided into the control group and the intervened group. Immunohistochemistry and realtime-PCR methods were used to measure the gene expression of ER alpha, IGF-1R and EGFR and their protein synthesis in epiphyseal growth plate.
After being intervened with NYRF, the gene expressions of ER alpha and IGF-1R were down-regulated and their protein synthesis markedly reduced, while those of EGFR were unchanged.
NYRF can modulate the development and maturation of bone by regulating the expressions of ER alpha and IGF-1R in the epiphyseal growth plate."
ERalpha inhibition may result in increased length but IGF1R knockout reduces height.
[Effect of Chinese herbs for nourishing shen-yin and removing xiang-fire on estrogen receptor expression in reproductive organ of rats contaminated with environmental endocrine disruptor].
"To investigate the effect of Chinese herbs for nourishing Shen-yin and removing Xiang-fire (NYRF) on estrogen receptor (ER) expression in uterus and ovary of rats contaminated with nonylphenol (NP) or its bisphenol A (BPA) mixture, for exploring the action mechanism of NYRF in antagonizing the estrogen-mimetic activity of environmental endocrine disruptors (EEDs).
EEDs contaminated female SD rats, 3-week old, were divided into two groups, the treated group fed with NYRF and the control group with corn oil during the same period of contaminating for 15 days. The wet weight (WW) and organ coefficient (OC) of uterus in rats, as well as the ER protein and mRNA expressions in rat's uterus and ovary were detected and compared.
As compared with normal range, WW and OC increased significantly in the contaminated rats of the control group, with significantly down-regulated ER protein expression in uterus, and expressions of ER alpha and ER beta gene and protein in ovary (P<0.05). While in the treated group, the above-mentioned abnormalities of various indictors were markedly reversed to a certain extent (P<0.05).
EEDs show estrogenic-mimetic action on productive organs, which could be antagonized by NYRF, resulting in the down-regulated mRNA and protein expressions of ER in reproductive organs, so as to reduce the sensibility of reproductive organs to EEDs, which is probably one of the acting mechanisms of NYRF."
Effect of nourishing “Yin”-removing “Fire” Chinese herbal mixture on hypothalamic kisspeptin expression in female precocious rats
"Both male and female rats show an obvious increase in Kiss-1 mRNA levels that coincides with the onset of puberty. Pharmacological analyses further set the contention that kisspeptin is likely the most potent elicitor of the GnRH/gonadotropic axis known so far"
"[YNRF] decreased the expression levels of GnRH, FSH and LH mRNAs in female adolescent rats"
"The mixture prescription is mainly composed of 10 medicinal plants: 15 g of Rehmannia glutinosa (Sheng di), 9 g each of Scrophularia buergeriana (Xuan shen), Anemarrhena asphodeloides (Zhi mu), Cortex Phellodendri (Huang bai), Paeonia suffruticosa Andr. (Dan pi), Alisma plantago-aquatica L. var. orientala Sam (Ze xie), Prunella vulgaris L. (Xia ku cao), 12 g of Carapax et Plastrum Testudinis (Gui jia), 30 g of Fructus hordei germinate (Mai ya) and 6 g of Gentiana scabra Bge. (Long dan cao)."
Included in Le Shu Yuan Jin Yin Hua tea.