Jaoga-Yukmiwon

Effects of Jaoga-Yukmiwon®, a Korean herbal medicine, on chondrocyte proliferation and longitudinal bone growth in adolescent male rats

"The effects of the Korean herbal medicine, Jaoga-Yukmiwon®, on the growth of adolescent rats were investigated in the present study. The proliferation ratio of chondrocytes was calculated from 5-bromo-2′-deoxyuridine incorporation during DNA synthesis. The 5-bromo-2′-deoxyuridine incorporation ratio of the control group was 21.1 ± 3.5%, and Jaoga-Yukmiwon® administration markedly increased the ratio to 34.5 ± 4.6% (p < 0.05). The bone formation rate of longitudinal bone was estimated by labelling with tetracycline, which binds to newly formed bone. The bone formation rate in the tibia of Jaoga-Yukmiwon®-administered rats was increased to 405.1 ± 4.3 µm (p < 0.05) from 292.2 ± 11.8 µm (control value). The height of the growth plate in the administrated rats was increased to 614.6 ± 10.0 µm (p < 0.05) from 552.3 ± 17.1 µm. The bone morphogenetic protein-2 immunostaining in the growth plate was also increased. These results suggest that Jaoga-Yukmiwon® may promote longitudinal bone growth during the developmental period."

"[JY] consists of the alcohol extract of seven medicinal plants, namely Acanthopanacis Cortex (Ja-Oh-Ga in Korean), Rehmanniae Radix Preparat (Suk-Ji-Hwang), Poria (Bok-Ryong), Dioscoreae Rhizoma (San-Yak), Corni Fructus (San-Su-Yu), Cervi Cornu (Nok-Kac) and Ginseng Radix (Go-Ryo-In-Sam). The main component of JY is Acanthopanacis Cortex which has been used for growth disorder in Korean medicine"

"Adolescent male Sprague-Dawley rats (3 weeks of age) were used in the experiment"

"Rats were divided into two groups (n = 8 in each group). The control group was given a daily oral administration (p.o.) of saline and the JY group was given a daily administration of JY (100 mg/kg, p.o.) for 5 consecutive days in each case.  On days 4 and 5 of experiment, all animals were injected
intraperitoneally with 10 mg/kg tetracycline for the measurement of bone growth. On day 6, BrdU
(30 mg/kg) was injected intraperitoneally 1 h before killing to label proliferating cells. One hour after the injection, animals were totally anaesthetized using ether and killed."