Monday, December 28, 2009

Huogo I Formula

Angelica Sinensis is available for sale Solaray - Dong Quai Angelica Sinensis, 550 mg, 100 capsules

Effects of huogu I formula (I) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head.

The microenvironment of a femoral head recently undergone necrosis may differ from an adult femoral head.

"Forty-eight healthy female Leghorn chickens were randomly divided into control group, model group and Huogu I group, and each group consisted of 16 chickens. At the meantime of model establishment, chickens of the Huogu I group were administrated with decoction, while the model and control group with distilled water by gavage. At the 8th and 16th week after medication, blood samples were obtained for blood lipid detection while both sides of femoral head were harvested for the rest of examinations. .
Compared with the control group, serum levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the model group rose significantly. Positive cell counting of BMP2, TGFβ(1), Smad4 and OPG in femoral head of the model group dropped prominently. Positive cell counting of Smad7 and RANKL increased dramatically. In contrast with the model group, levels of TC, TG and LDL-C in Huogu I group reduced significantly. Positive cell counting of BMP2, TGFβ(1), Smad4 and OPG in femoral head of the Huogu I group increased prominently. Indices of Smad7 and RANKL both decreased significantly. Especially at the 8th week, these variations were more significant.
Huogu I formula is effective in promoting repair of necrotic femoral head by regulating the expressions of BMP2, TGFβ(1), Smads and OPG/RANKL of osteoclast in femoral head."

"Huogu Ⅰ Formula (consisting of Poria Cocos, Atractylodes Rhizome, Codonopsis Pilosula, prepared Pinellia tuber, Radix Paeonia Rubra, Ramulus Cinnamoni, Angelica Sinensis, Ligustici Chuanxiong and prepared Rhizome Rehmannia) provided by Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, was decocted into solution which contained crude drug in 1 g/mL. Methyl prednisolone sondium succinate"

The model group refers to the osteonecrosis group, the control had no stimulation, and the Huogo I group is Huogo I +necrosis.

"Eight weeks after steroid administration, the model group showed several histomorphological changes as compared with the control group: thin trabecula, increased number of empty lacuna and adipocyte proliferation and hypertrophia. At the 16th week, collapsed bone trabeculae, necrosis accompanied by hyperplasia in some regions, increased empty lacuna were observed. Moreover, the fat area in marrow cavity was higher than that in the normal group. At the 8th and 16th week after treated by Huogu Ⅰ Formula, trabeculae were arranged in regular and compact shapes, large amount of osteoclast and few osteoclast distributed around them, and the empty lacuna rate and fat area in medullary channel were significantly lower than those in the model group"

"The expressions of BMP2 and TGFβ1 positive staining were observed in chondrocye and osteocyte of femoral head in the control group. At the 8th week, the expressions of BMP2 and TGFβ1 in the model group were significantly lower than that in the control group. Additionally, the difference was even more obvious at the 16th week. Compared with the model group, the expressions of BMP2 and
TGFβ1 in Huogu Ⅰ group were significantly higher. At the 8th week, the increasing trend of the two factors in Huogu Ⅰ group was drastic and even approximate to counterparts in the control group{but not greater than the control group which is what we would hope if Huogo I formula is height increasing}"

"Rhizoma Chuanxiong is effective in improving microcirculation and reducing the blood viscosity; Atractylodes Macrocephala Koidz, Angelica Sinensis, and prepared Pinellia Tuber possess similar effects and may improve the condition of hyperlipidemia and prevent fat from accumulating in marrow cavity."

"Angelica Sinensis possesses effects of up-regulating expressions of BMP2, cbfal, insulinlike growth factors (IGF), and other related genes of osteoblast{many of these genes are pro-chondrogenic also}. We believe that, on one hand, Huogu Ⅰ Formula acts on osteoblast and bone marrow stromal cell to enhance the expression of related genes of osteogenesis. On the other hand, it regulates the lipid metabolism and improves the condition of abnormal blood rheology, leading to the promotion of blood supply of femoral head."<-So Angelica Sinensis is the most promising compound.

Study to establish the role of JAK2 and SMAD1/5/8 pathways in the inhibition of hepcidin by polysaccharides from Angelica sinensis., states that Angelica Sinensis may inhibit the SMAD1/5/8 pathway.  Smad1/5/8 is the BMP pathway signaling.

Role of Bone Morphogenetic Proteins-7 (BMP-7) in the Renal Improvement Effect of DangGui (Angelica sinensis) in Type-1 Diabetic Rats., states that Angelica sinensis may directly activate BMP-7.

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