Restoration of longitudinal growth by bioengineered cartilage pellet in physeal injury is not affected by low intensity pulsed ultrasound
"We investigated the application of a Bioengineered Cartilage Pellet (BCP) in repairing a rabbit physeal fracture model, and the possible effects of Low Intensity Pulsed Ultrasound (LIPUS) treatment. Rabbits with physeal fracture created were assigned to the NC group (no BCP, no LIPUS), GC group (BCP, no LIPUS), and GT group (BCP and LIPUS). Femoral lengths and cartilage area were assessed at 4, 8, and 16 weeks post-defect. After transplantation, the BCP showed continuous growth in the host and demonstrated resemblance to a natural growth plate. The GC group showed 34.1, 32.1, and 41.1% advantage in lengthening over the NC group and the GT group showed 51.1, 41.6, and 26.9% improved lengthening than the NC group, at 4 (p = 0.203), 8 (p = 0.543) and 16 weeks (p = 0.049), respectively. Cartilage area was shown to be significantly higher in GC and GT group compared to NC group (p < 0.05). No significant difference was found between GC and GT group. Femoral longitudinal growth was shown to be improved by the BCP, however no additional enhancement effect was shown to be provided by LIPUS[maybe they targeted LIPUS on the wrong cells or the LIPUS stimulation wasn't enough?]. "
Here's the study that describes how pellet culture was performed unfortunately I don't have access to it:
Here's another study however that explained the method to form chondrocyte pellets:
In the bone marrow there are no chondrocytes and thus no cartilagenous matrix to digest. The medium is mostly things that already exist in the bone marrow. What we can do is use centrifugal force to get more mesenchymal stem cells in one location. The development of cartilage has been shown to be initiated by a condensation of mesenchymal precursor cells. Maybe Kojima's rotation device can help you grow taller... Kojima's green light could demineralize a bone surface and then the rotation could get a bunch of stem cells onto that bone surface.
So maybe after LSJL you need to use a bicycle to get stem cells onto a bone surface which has been demineralized by hydrostatic pressure.