Wednesday, August 18, 2010

Increase non-long bones height & long bones height with puerarin?

Non-long bones grow differently than long bones do but there are some bones with the properties of both.  The mandible is an irregular bone but grows like a long bone in some places(you can still get a bigger chin though) and the distal phalanges have the ends covered by periosteum so they can also grow in length by increasing cortical bone formation.

And of course increased cortical bone formation will increase height in the vertebrae(the spine).  So, is puerarin a possible substance that can be issued to increase spinal height amongst other things? 

Stimulatory effect of puerarin on bone formation through activation of PI3K/Akt pathway in rat calvaria osteoblasts. 

"Puerarin, a natural isoflavonoid found in Chinese Pueraria lobata (Wild.) Ohwi, has received increasing attention because of its possible role in the prevention of osteoporosis.  Rat osteoblasts isolated from newborn Wistar rats were used to investigate the effect of puerarin on osteoblasts[remember cells used outside the body can respond differently than inside the body due to lack of negative feedback[, and its possible molecular mechanism. Data showed that puerarin caused a significant increase in cell viability, alkaline phosphatase (ALP) activity and mineral nodules formation in osteoblasts, suggesting that puerarin had a stimulatory effect on osteoblastic bone formation. This functional improvement by puerarin was accompanied by activation and nuclear translocation of Akt. Furthermore, puerarin-stimulated osteoblastic growth, Akt activation and redistribution were significantly blocked by the specific PI3K inhibitor, LY294002. These results strongly suggested that puerarin stimulated osteoblastic proliferation and Akt activation in a PI3K-dependent manner. In summary, puerarin derived from Chinese Pueraria lobata (Wild.) Ohwi can promote bone formation in cultured rat osteoblasts, which might be mediated by activation of the PI3K/Akt pathway. DMEM:Dulbecco's modification of Eagel's medium PBS:phosphate buffered saline DMSO:dimethyl sulfoxide EDTA:ethylene diamine tetraacetic acid SDS:sodium dodecyl sulfate SDS-PAGE:sodium dodecylsulfate polyacrylamide gel electrophoresis FITC:fluorescein isothiocyanate HRP:horseradish peroxidase PI3K:phosphatidylinositol 3-kinase." 

The PI3K pathway is a very important regulator of cellular proliferation so it has the potential to increase height anywhere, even in long bones(as long as there are actively proliferating chondrocytes in the epiphyseal hyaline cartilage). 

Effect of puerarin on bone formation. 

Puerarin is one of the major phytoestrogens isolated from Pueraria lobata, a Chinese medicine known as Gegen. Our laboratory compared the amount of new bone produced by puerarin in collagen matrix (carrier) to that produced by the collagen matrix alone. 
Eighteen bone defects, 5mm by 10mm were created in the parietal bone of nine New Zealand White rabbits. In the experimental group, six defects were grafted with puerarin solution mixed with collagen matrix. In the control groups, six defects were grafted with collagen matrix alone (active control) and six were left empty (passive control). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Serial sections were cut across each defect. No new bone was formed in the passive control group. Quantitative analysis of new bone formation was made on 100 sections (10 sections in each defect, in five defects randomly selected in each of the experimental group and active control group). 
A total of 554% more new bone was present in defects grafted with puerarin in collagen matrix than those grafted with the collagen matrix alone. 
Puerarin in collagen matrix has the effect of increasing new bone formation locally and can be used for bone grafting or for bone induction often required in surgery."

Puerarin stimulates osteoblasts differentiation and bone formation through estrogen receptor, p38 MAPK, and Wnt/β-catenin pathways.

"Puerarin is an isoflavone extracted from Radix Puerariae, a traditional Chinese herb used to treat many diseases such as osteoporosis. In this study, puerarin was shown to stimulate alkaline phosphatase (ALP) activity, type I collagen (Col I) secretion, and mineralized nodules formation of primary osteoblasts. Whereas the estrogen receptor (ER) antagonist ICI 182780 was able to reduce the increase in ALP activity and Col I secretion induced by puerarin{if estrogen receptor levels are low level than puerarin may become more chondrogenic}. Furthermore, puerarin was shown to elevate levels of phospho-p38 mitogen-activated protein kinase (MAPK) and β-catenin proteins in a time-dependent manner. Pretreatment of osteoblasts with ICI 182780 can reduce this elevation, whereas pretreatment with p38 MAPK inhibitor SB 203580 did not affect the increase of β-catenin protein. Meanwhile, intragastric administration of puerarin protected against reduction in bone mineral density and bone mineral content in ovariectomized rats, and improved femur trabecular bone structure. Taken together, ER, p38 MAPK, and Wnt/β-catenin pathways were involved in puerarin-stimulated osteoblasts differentiation and bone formation."

Some studies have suggested that Puerarin can reduce TGF-Beta1 expression but those studies have not been in bone.

Stimulatory effect of puerarin on bone formation through co-activation of nitric oxide and bone morphogenetic protein-2/mitogen-activated protein kinases pathways in mice.

"Osteoblast cells were harvested from 8-month old female imprinting control region (ICR) mice. The effects of puerarin stimulation on the proliferation, differentiation and maturation of osteoblasts were examined. The production of nitric oxide (NO) and the expression of bone morphogenetic protein-2 (BMP-2), SMAD4, mitogen-activated protein kinases (MAPK), core binding factor α1/ runt-related transcription factor 2 (Cbfa1/Runx2), osteoprotegerin (OPG), and receptor activator of NF-κB ligand (RANKL) genes were analyzed. The activation of signal pathways was further confirmed by specific pathway inhibitors.
The osteoblast viability reached its maximum at 10(-8) mol/L puerarin. At this concentration, puerarin increases the proliferation and matrix mineralization of osteoblasts and promotes NO synthesis. With 10(-8) mol/L puerarin treatment, BMP-2, SMAD4, Cbfa1/Runx2, and OPG gene expression were up-regulated, while the RANKL gene expression is down-regulated. Concurrent treatment involving the (bone morphogenetic protein) BMP antagonist Noggin or the NOS inhibitor L-NAME diminishes puerarin induced cell proliferation, Alkaline phosphatase (ALP) activity, NO production, as well as the BMP-2, SMAD4, Cbfa1/Runx2, OPG, and RANKL gene expression.
In this in vitro study, we demonstrate that puerarin is a bone anabolic agent that exerts its osteogenic effects through the induction of BMP-2 and NO synthesis, subsequently regulating Cbfa1/Runx2, OPG, and RANKL gene expression"

"For osteoblasts, puerarin had significant stimulatory effects on the ERK1/2, and p38 activation at 5, 15, and 30 minutes and on JNK activation at 5 and 15 minutes. We saw no effect on ER-α activation, while treatment with puerarin stimulated ER-β at 5, 15, 30, 60, 120 and 180 minutes. Puerarin was also able to up-regulated levels of BMP-2 protein at 60 and 120 minutes"

"Noggin, the bone morphogenetic proteins antagonist, and L-NAME, the NOS inhibitor, blocked puerarin-mediated cell proliferation, ALP secretion, and NO production"

Now all of the positive benefits of puerarin on bone studies seem to come from China so there might be a possibility of bias(puerarin is a part of CTM).  Puerira Iobata is also known at Kudzu and is available for sale like here at Planetary Herbals Full Spectrum Kudzu, 750 mg, Tablets , 240 tablets.


  1. If this is for real I'm shitting bricks with excitement. I live in Georgia and there's so much Kudzu here they have to poison it just to keep it from eating peoples houses. Hell, I'm looking at it right outside my window. Maybe I'll start making salads outta this stuff.

  2. Well, if there's so much of the stuff then there's not much opportunity cost in terms of taking it(cash). I can't really recommend doses, I can only say whether the research shows whether this substance or chemical has the potential to increase height or not.

    And yes Kudzu does have potential to be used to increase height.

  3. then again none of the experiments involve ingesting of Puerira Iobata? so its hard to say if this will work towards height increase.
    Aniway, hows LSJL doing for you tyler?

  4. First poster here, i could chew it like tobacco which would essentially absorb the chemicals inside the plant directly into my bloodstream or use the same method with an essential oil distillation to make it easier.

  5. Hey TCD, check out this link, this guy says hes had 1.15 in sucess in 2 months with puerarin.

    My account on this site is Sinister Psyche btw. You oughta make an account there and get more people to come to your blog.

  6. Pueraria is used by the ladies to grow breasts.

  7. Can puerarin cause acromegaly?