Friday, October 19, 2012

Grow with CLA?

MRM CLA 1250 High Potency,180 Softgels

CLA will help you grow taller with open growth plates due to IGF-1 and CTGF.  However, with trying to induce new growth plates by LSJL it's unclear as CLA has anti-inflammatory effects and inflammatory factors are likely needed to induce new growth plate formation.  But with open plates, chondrocyte hypertrophy is the predominant determinant of height increase so any stimulatory effects on chondrocyte hypertrophy outweigh any other inhibitory effects on other stages.  However with closed plates without first stages like mesenchymal condensation and initial differentiation into chondrocytes, you won't grow taller despite chondrocyte hypertrophy benefits.

Gene expression profiles in rat mesenteric lymph nodes upon supplementation with conjugated linoleic acid during gestation and suckling.

"Human milk contains conjugated linoleic acid (CLA), a fatty acid.
The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip(®) Rat Genome 230 2.0 (Affymettrix). {We identify} 89 genes differentially expressed in all three dietary approaches. Several genes, such as connective tissue growth factor (Ctgf){CTGF binds to Nov and CTGF has pro chondrogenic effects}, tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), [are] highly interconnected nodes of [a] resulting network."

"Ctgf, Timp1, Gal and Syt1 are genes modulated by CLA supplementation."

"Ctgf promotes DNA synthesis in chondrocytes, osteoblasts and fibroblasts"

This next study provides explanation as to whether CLA is needed when one is already taking colostrum.  Remember CLA is in human milk and not bovine milk.

Effects of conjugated linoleic acids fed to dairy cows during early gestation on hematological, immunological, and metabolic characteristics of cows and their calves.

"The aim of the present experiment was to test the stimulation ability of peripheral blood mononuclear cells (PBMC) expressed as stimulation index (SI) of newborn calves and of their dams fed a control fat supplement (CON) or 50 and 100g/d of a CLA-containing fat supplement (CLA50 and CLA100, respectively) during the preceding lactation period for 182 d after calving. The total intake of cis-9,trans-11 and trans-10,cis-12 CLA by groups CLA50 and CLA100 amounted to 4 and 8 g/d each, respectively. For this purpose, blood was collected immediately after parturition from calves before and after colostrum intake, and from cows after parturition and 21 d later. The SI was related to the fatty acid composition of erythrocyte and milk lipids and to various hematological and clinical-chemical parameters. Retrospective evaluation revealed that depletion time (i.e., the individual period elapsed between the day of terminating the feeding of the experimental diet in the preceding lactation period and the day of calving) ranged from 190 to 262 d, which corresponded to fetal exposure times of 19 to 102 d. The SI from cows increased significantly by 77 and 55%, within 21 d after calving according to the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) and Alamar Blue assays, respectively.  Feeding of 50 g of the CLA product failed to demonstrate this increase. Moreover, SI was significantly lower for calves whose dams belonged to the CLA50 group, whereas stimulation ability was comparable for the PBMC from calves whose mothers were treated with CON and CLA100. Plasma metabolites (total bilirubin, total cholesterol, glucose, nonesterified fatty acids, 3-β-hydroxybutyrate, total protein, and albumin) and hematological parameters (hematocrit, white blood cell profile) were not significantly influenced by dietary treatments of the cows in the preceding lactation period. Although the fatty acid pattern of erythrocyte lipids of cows remained uninfluenced, that of calves showed alterations due to the feeding type of their dams. For example, C16:0 increased significantly from 14.4 to 16.9% of total fatty acid methyl esters, whereas cis-9,trans-11 CLA increased slightly from 0.11 to 0.15% at the same time in calves when their mothers were fed the CLA100 instead of the CON diet. Fatty acid profile of colostrum was significantly different from that of milk after 3 wk for most of the detected fatty acids, but was not influenced by diet type.  feeding a CLA-containing fat supplement during the preceding lactation and gestation period exerted effects on the stimulation ability of PBMC from cows and calves for the subsequent parturition."

"[There were] long-term effects of CLA feeding on cows and their offspring."<-but we don't know the effects on chondrocytes and bone.

"Inflammatory cytokines inhibit chondrocyte proliferation and induce cartilage degradation for which part of the response is mediated by PGE2. Excess production of PGE2 is associated with bone and proteoglycan loss. PGE2 influences the IGF-I/IGFBP axis to facilitate bone and cartilage formation. Growing rats given butter fat and supplements of CLA demonstrated an increased rate of bone formation and reduced ex vivo bone PGE2 production, respectively. The supplements of CLA isomers resulted in their enrichment in lipids of various bone compartments of animals. The effects of CLA on bone biology in rats (IGF action and cytokines) appear to be dependent on the level of n-6 and n-3 fatty acids in the diet;  CLA [also decreases] ex vivo bone PGE2 production and in osteoblast-like cultures.  CLA, may be beneficial in moderating cyclooygenase 2 (COX-2) activity or expression (influencing PGE2 biosynthesis)."

Inflammation may be pro-differentiation.  Distraction Osteogenesis involves inflammation after all.

"CLAs occur naturally in ruminant food products (beef, lamb and dairy) because of the process of bacterial biohydrogenation of linoleic acid in the rumen"

"The anabolic effects of PGE2 may occur through stimulation of osteoblast endogenous IGF-I production or by increasing bone cell responsiveness to IGF-I."

"chondrocytes are either sensitive to excess n-6 fatty acids or to an overproduction of PGE2. Growth cartilage in children and young animals contains small amounts of n-6 fatty acids, but a relatively high concentration of 20:3(n-9) (Mead acid)"

CLA reduced liver IGF-1 levels possibly a negative feedback mechanism.

Evaluation of the mechanism of action of conjugated linoleic acid isomers on reproduction in dairy cows. stated that CLA increased circulating IGF-1 levels.  Supplementation occurred for 37 days.  cis-12 CLA (CLA 50:50) resulted in greatest IGF-1 levels.

1 comment:

  1. Longitudinal shrinkage in lower legs: "Negative growth" in healthy late-adolescent males