Friday, January 9, 2009

Chitanase 3-like protein's

LSJL downregulates CHI3L3.

Human YKL39 (chitinase 3-like protein 2), an osteoarthritis-associated gene, enhances proliferation and type II collagen expression in ATDC5 cells.

"Human YKL39 (chitinase 3-like protein 2 /CHI3L2) is a secreted 39 kDa protein produced by articular chondrocytes and synoviocytes. hYKL39 expression is increased in osteoarthritic articular chondrocytes. Sequence analyses indicated that hYKL39 has significant identity with the human chitotorisidase family molecules, although it is considered that hYKL39 has no enzymatic activity since it lacks putative chitinase catalytic motif. We overexpressed hYKL39 in ATDC5{chondrocyte progenitor cell line} cells. hYKL39 enhances colony forming activity, cell proliferation, and type II collagen expression in these cells."

"overexpression of hYKL39 enhanced cell proliferation by around 3-fold [and increased the percentage of cells in the S-phase]"

"expression of Cyclin D1, whose expression level is known to increase transiently in late G1 phase, was significantly increased in hYKL39 overexpressing cells"

"DDR1 expression is significantly increased in the hYKL39 overexpressing cells while integrin beta-1 and beta-3 expression was significantly decreased"

"Chitin is a long-chain biopolymer of N-acetylglucosamine (GlcNAc) similar to the structure of cellulose"

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