Saturday, January 17, 2009

What's the typical gene expression for the epiphysis of human long bones?

Since the goal of LSJL is to induce chondroinduction in the epiphysis of the long bone for new height gain.  Note that growth plates do not have to be a linear straight line like in the epiphyseal plate they can be round within the epiphysis.

Expression of genes for bone morphogenetic proteins BMP-2, BMP-4 and BMP-6 in various parts of the human skeleton.

"Seven bone samples recovered from various parts of skeletons from six cadavers of young healthy men who died in traffic accidents were collected. 
Expression of m-RNA for BMP-2{up in LSJL} and BMP-4 is higher in trabecular bone in epiphyses of long bones, cranial flat bones and corpus mandibulae then in the compact bone of diaphyses of long bones. BMP-4 was higher [in all samples] than for BMP-2.
BMP-6 is not expressed in the collected samples at all."

So if BMP-6 is important for chondrogenesis that could be a problem.  Another issue could be if BMP-2 needs to be at higher levels than BMP-4 to be permissive for cartilage growth.

"In bone matrix BMPs are connected with collagen and are not active unless released by the action of collagenases of osteoclasts"<-Thus degradation of bone matrix could play a key role in chondroinduction by LSJL.

"Biopsies (5–7 cm long and 2–3 cm wide) were taken from seven different bones. Bone marrow was removed from trabecular bone"

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