Monday, November 29, 2010

Boosting Height with GHenerate?

Several people on the grow tall forum have reported height increase with GHenerate(LG Science GHenerate Spray, 4-Ounce-if you want to try please take before and after pics).  GHenerate, which is produced by LGSciences, contains puerarin which is a strong bone growth and PI3K pathway stimulator.  PI3K is a potent anabolic stimulus.  Puerarin stimulates osteoblasts and may increase height if the osteoblasts decide to deposit new bone on the surface of the long bones.  PI3K is also anabolic to chondrocytes but I don't see how a PI3K stimulator alone is making people 1 to 2 inches taller in such a short time frame(the claims are not supported by photographic evidence of any kind, PI3K stimulators should result in thicker bones).  PI3K stimulation can help grow bone during puberty as well...

PI3K/Akt signaling as a key regulatory pathway for chondrocyte terminal differentiation.

"Phosphoinositide-3 kinase (PI3K) and its major downstream molecule, Akt, a serine-threonine kinase, play pivotal roles in [chondrogenesis]. Akt signaling was activated in resting and proliferative chondrocytes but was reduced during terminal differentiation. We adopted two chondrocyte differentiation systems to investigate the roles of PI3K/Akt signaling in chondrogenesis. First, we employed an embryonic forelimb organ culture of transgenic mice expressing an Akt-Mer (a ligand-binding domain of a mutated estrogen receptor) fusion protein whose kinase activity was conditionally activated by treatment with 4-hydroxytamoxifen (4OHT). Activation of Akt signaling in embryonic chondrogenesis enhanced chondrocyte proliferation and inhibited hypertrophic differentiation, presumably due to the suppressed expression of Runx2, a transcription factor critical for chondrocyte terminal differentiation. Conversely, inhibition of PI3K by its inhibitor accelerated terminal hypertrophic differentiation, resulting in a shorter bone. Essentially the same results were obtained in a second line of experiments using human synovial stromal cells (hSSCs), which are mesenchymal progenitor cells isolated from adult joints.  PI3K/Akt signaling is a key regulator in terminal chondrocyte differentiation in both embryonic and adult chondrogenesis."

PI3K regulates Akt which slows down terminal differentiation of chondrocytes.  Increasing PI3K and in turn Akt will result in more height growth by allowing for more proliferative cycles before terminal chondrocyte differentiation.  That would not explain how people are growing post epiphyseal and diaphyseal fusion.  Perhaps PI3K interacts with DNA Methylation in some way allowing for a rebirth of chondrocytes.

Vitamin D receptor expression is associated with PIK3CA and KRAS mutations in colorectal cancer.

"The vitamin D receptor (VDR) is a transcription factor, which plays an important role in cellular differentiation and inhibition of proliferation[VDR reduces height gain]. A link between VDR and the RAS-mitogen-activated protein kinase (MAPK) or phosphatidylinositol 3-kinase (PI3K)-AKT pathway has been suggested. However, the prognostic role of VDR expression or its relationship with PIK3CA or KRAS mutation remains uncertain. Among 619 colorectal cancers in two prospective cohort studies, 233 (38%) tumors showed VDR overexpression by immunohistochemistry. We analyzed for PIK3CA and KRAS mutations and LINE-1 methylation by Pyrosequencing, microsatellite instability (MSI), and DNA methylation (epigenetic changes) in eight CpG island methylator phenotype (CIMP)-specific promoters [CACNA1G, CDKN2A (p16), CRABP1, IGF2, MLH1, NEUROG1, RUNX3, and SOCS1] by MethyLight (real-time PCR). VDR overexpression was significantly associated with KRAS mutation (odds ratio, 1.55; 95% confidence interval, 1.11-2.16) and PIK3CA mutation (odds ratio, 2.17; 95% confidence interval, 1.36-3.47), both of which persisted in multivariate logistic regression analysis. VDR was not independently associated with body mass index, family history of colorectal cancer, tumor location (colon versus rectum), stage, tumor grade, signet ring cells, CIMP, MSI, LINE-1 hypomethylation, BRAF, p53, p21, beta-catenin, or cyclooxygenase-2. VDR expression was not significantly related with patient survival, prognosis, or clinical outcome. In conclusion, VDR overexpression in colorectal cancer is independently associated with PIK3CA and KRAS mutations. Our data support potential interactions between the VDR, RAS-MAPK and PI3K-AKT pathways."

The PI3K pathway may alter the Methylation status of the Vitamin D Receptor allowing for further growth after puberty.

The next ingredient of Ghenerate is a peptide in Marus Alba that mimics the effects of GHRP and Ghrelin(Ghrelin is a growth factor for osteoblasts and chondrocytes).

Ghrelin in plants: what is the function of an appetite hormone in plants?

"The expression of a ghrelin-like peptide hormone that has only been associated with animals, in various plant tissues. Ghrelin, the appetite stimulating hormone, has been identified from a number of different species including humans, rat, pig, mouse, gerbil, eel, goldfish, bullfrog and chicken. The study here was conducted using an immunohistochemistry assay to screen whether plants have any ghrelin immunoreactivity. In this respect, Prunus x domestica L. and Marus alba were examined. Immunohistochemistry results showed that there is a strong human ghrelin immunoreactivity substance in the parenchyma cells of these plants. This was entirely unexpected since this hormone was considered to be present solely in animals. Thus, this study is the first to report the presence of a peptide with ghrelin-like activity in plants, a finding that has only been observed in the animal kingdom. RIA analysis confirmed that these plants contain significant amounts of this substance. Furthermore, reverse-phase HPLC analyses of plant extracts showed an elution characteristic of the peptide identical to that of human ghrelin. In general, fruit from both plants had higher levels of the peptide than the vegetative parts."

This is the only study on the site regarding Marus Alba(could be actually Morus Alba).  We don't know whether consuming exogenous Ghrelin in plant form will result in higher serum levels in humans or whether this Ghrelin will be effective.

My advice skip Ghenerate and save money by buying plain puerarin.


  1. is puerarin something you can personally see yourself doing in the future?

  2. hmm i also thought about buying peuperin bulk kilo cheap from alibaba,
    im not sure but i searched for the mentioned plants which had the ghrelin like plant peptides and i found white mulberry fruit and plums, in the study i read they also mentioned the fruit of the plant being more concentrated in the ghrelin compounds, so maybe you could just buy peuperin (80 or 90% at alibaba) and those fruits.

  3. i can buy puerarin in powder form but i don't know how should i take it or even how much will i take it. Can you give me your advice ?

  4. you should search for studies online and how much the researchers are using

    also you want to take breaks because it may weaken the effect to continually use peuperin..

  5. I tried to read this but none of that made sense to me